Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human TAOK1 (1-314) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs) (1). The activation of and binding to MEK3 by TAOK1 implicates TAOK1 in the regulation of the p38-containing stress-responsive MAP kinase pathway. A microtubule affinity-regulating kinase kinase, TAOK1 (also known as MARKK) is an important regulator of mitotic progression, required for both chromosome congression and checkpoint-induced anaphase delay (2). Kinase Enzyme System contains: Kinase: TAOK1, 10ug (Human, recombinant; amino acids 1-314). MW: ~63kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. TAOK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/57551/.
