BIO Q Membrane Filter-Hydrophilic PTFE, 0.45um, 13mm
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The SignaTECT Protein Kinase Assay Systems contain the proprietary SAM2 Biotin Capture Membrane. The streptavidin-coated SAM2 Membranes possess high binding capacity and high specificity characteristics, producing lower backgrounds and higher signal-to-noise ratios compared to the P81 phosphocellulose method of capture and measurement. The perforated and numbered membrane allows measuring from 1 up to 96 kinase reactions in less than 1 hour. Following the kinase reaction, spot samples onto the SAM2 Membrane and perform a series of short wash steps to remove nonspecific label. Use the SAM2 Membrane under a variety of buffer/reaction conditions (e.g., cell extracts), not allowed by many other methods. Lastly, the high binding capacity allows use of the SignaTECT Systems for kinetic studies. Each system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as the necessary reaction components. The researcher must supply [gamma-32P]ATP.
