Bio-Nobile 96-M Robotic Workstation
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
psiCHECK-2 Vector provides a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla luciferase is used as the primary reporter gene; the gene of interest is cloned into a multiple cloning region located downstream of the Renilla translational stop codon. Initiation of the RNAi process by synthetic siRNAs toward a gene of interest results in cleavage and subsequent degradation of the fusion mRNA. Measuring decreases in Renilla activity provides a convenient way of monitoring the RNAi effect. In comparison with other fusion approaches, the Renilla luciferase approach offers more convenient and rapid quantitation with higher sensitivity. The psiCHECK-2 Vector contains a second reporter gene, firefly luciferase, and is designed for endpoint lytic assays.
