Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. Based on 2 rare-cutting restriction enzymes, SgfI and PmeI, it provides rapid, efficient and high-fidelity transfer of protein-coding regions between a variety of Flexi Vectors without resequencing. pFN9A Vector is designed specifically for high-level protein expression in mammalian cells from the CMV promoter and provides Renilla luciferase, which may be used as a transfection control. This vector complements pGL4 firefly luciferase vectors when exogenous proteins (e.g., a receptor or transcription factor) must be expressed for reporter assays. All inserts may be confirmed by cell-free expression with the TNT T7 Quick System. Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during subcloning. The Flexi System replaces the barnase gene with your insert. This vector cannot be cultured in normal laboratory strains of E. coli without an insert.
