Bioneer DNA/RNA Preparation Kits

PowerPlex Fusion 6C System is a 27-locus multiplex for human identification applications. This 6-color system allows co-amplification and fluorescent detection of the 18 autosomal loci in the expanded CODIS core loci (CSF1PO, FGA, TH01, vWA, D1S1656, D2S441, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433 and D21S11) and Amelogenin and DYS391 for gender determination. Penta D, Penta E, TPOX, D22S1045 and SE33 loci also are included to increase discrimination and allow database searching. Finally two rapidly mutating Y-STR loci, DYS570 and DYS576, are in the multiplex. The system works well with extracted DNA samples, including low amounts of template DNA, mixtures and inhibitor-laden samples. Also use with direct amplification, enabling streamlined STR databasing efforts. Amplification is successful with FTA card punches, pretreated swabs, Bode Buccal DNA Collector or S&S 903 punches. Fast cycling conditions reduce sample-processing time.