RACK 80 WELL FL ASTD 5RK/PK 4PK/CS
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human PKC mu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C mu is a member of the protein kinase C (PKC) family that differs from the other PKC isoenzymes in structural and enzymatic properties. PKC mu is ubiquitous in nature with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKC mu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKC mu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). Kinase Enzyme System contains: Kinase: PKC mu, 10ug (Human, recombinant full-length). MW: ~131kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acids 109-121). Other: Reaction Buffer, DTT. PKC mu NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5587/.
