Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The vectors are for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using T7 RNA polymerase promoter. The GST tag boosts tagged protein solubility during E. coli expression. Flexi Vector System is a simple, directional cloning method for protein-coding sequences. Based on 2 rare-cutting restriction enzymes, SgfI & PmeI, it provides rapid, efficient and high-fidelity transfer of protein-coding regions between Flexi Vectors without resequencing. Direct transfers can only occur between 2 N-terminal tagged vectors or from an N-terminal to a C-terminal vector. pFN2A/K (GST) Flexi Vectors are designed for protein expression with an N-terminal GST tag in E. coli and T7 cell-free expression systems. Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during subcloning. The Flexi System replaces the barnase gene with your insert. These vectors cannot be cultured in normal laboratory strains of E. coli without an insert.
