Applied Separations Solid Phase Extraction (SPE)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhPAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 is one of the Group I PAKs, which also include PAK2 and PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2). System contains: Kinase: PAK1/CDC42, 10ug (Human, recombinant full-length). MW: ~96kDa (PAK1). Substrate: PAKtide (RRRLSFAEPG). Reaction Buffer, DTT, GTP Solution. PAK1/CDC42 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5058/.
