Applied Separations Solid Phase Extraction (SPE)

Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PAK1 has been activated in vivo. PAK1 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Kinase Enzyme System contains: PAK1 Kinase, 10ug (Human, full-length recombinant). MW: ~95kDa. PAKtide (1mg/ml), (RRRLSFAEPG). 5X Reaction Buffer, 0.1M DTT.