Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli, including growth factors, cytokines and phorbol esters. p70S6Kb contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. PI3 kinase pathway and mTOR are involved in the activation of p70S6Kb, but other pathways can also activate this target protein. Kinase Enzyme System contains: Kinase: p70S6Kb, 10ug (Human, recombinant full-length). MW: ~85kDa. Substrate: RSK substrate (KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acids 230-239). Other: Kinase Assay Buffer I, DTT. p70S6Kb NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6199/.
