Bioneer AccuRapid Cell-Free Protein Expression Kit
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Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation, and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylation at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine-389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). Kinase: p70S6K, 10ug (Human, recombinant full-length). MW: ~76kDa. Substrate: S6K substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acid 230-238). Other: Reaction Buffer, DTT. p70S6K NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6198/.
