Harvard Locomotor Activity and Exploration
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Full-length rhNUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2. NUAK2 is activated by muscle contraction and a unique mediator of contraction-stimulated glucose transport in skeletal muscle. NUAK2 is involved in cellular stress responses linked to obesity and type 2 diabetes. NUAK2 shows kinase activity against a synthetic test peptide and the activity in keratinocytes is increased by AMP and 5-amino-4-imidazolecarboxamide riboside, implying that AMPK kinase-dependent pathway can activate NAUK2. Glucose deprivation also increases NAUK2 activity in baby hamster kidney fibroblasts. Contains: Kinase: NUAK2, 10ug (Human, recombinant full length). MW: ~110kDa. Substrate: CHKtide (KKKVSRSGLYRSPSMPENLNRPR); derived from the human CDC25C protein isoform A (amino acid 205-225). Reaction Buffer, DTT. NUAK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/81788/.
