CyBio Drop automated liquid handler
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Recombinant human NEK9 (1-979; deleted fragment of 346-731) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. Kinase Enzyme System contains: NEK9 Kinase, 10ug (Human, recombinant; amino acids 1-979; deleted fragment of 346-731). MW: ~115kDa. Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml). 5X Reaction Buffer, 0.1M DTT.
