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Full-length rhNEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all proliferating cell lines; the amount does not change during the cell cycle. During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in CHO cells increases cytoskeletal re-organization in response to Prolactin, while downregulation of NEK3 expression by siRNA blocks these effects. Prolactin also stimulates interaction between NEK3 and paxillin, leading to increased paxillin phosphorylation. Analysis of breast tissue microarrays show a significant upregulation of NEK3 expression in malignant versus normal specimens. Contains: NEK3, 10ug (Human, recombinant full length). MW: ~86kDa. Substrate: Native Swine MBP. Reaction Buffer, DTT. NEK3 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4752/.
