Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
These products are for use with NanoBRET Target Engagement Kinase HDAC and BET BRD Assays. The NanoBRET TE Assay uses four key components: an expressed cellular target protein fused to the NanoLuc luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc luciferase; and a cell-impermeable inhibitor for NanoLuc luciferase. Bioluminescence resonance energy transfer is achieved by transferring the luminescent energy from NanoLuc luciferase to the fluorescent tracer bound to the target protein-NanoLuc fusion. Compounds that are applied to the cells and specifically engage the intracellular target protein-NanoLuc fusion will result in a decrease in BRET. To ensure accurate assessment of intracellular target engagement, a NanoLuc inhibitor is used to mitigate any extracellular NanoLuc signal that may arise from cells compromised during handling, while not adversely affecting NanoLuc luciferase expressed within healthy living cells.
