CyBio Drop automated liquid handler
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The NanoBRET Target Engagement (TE) Intracellular HDAC Assay measures compound binding at select HDAC target proteins within intact cells. Based on the NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells, the NanoBRET TE HDAC Assay analyzes the apparent affinity of test compounds by competitive displacement of a NanoBRET tracer reversibly bound to a NanoLuc HDAC fusion protein in cells. Bioluminescence resonance energy transfer is achieved by transferring luminescent energy from NanoLuc luciferase to the fluorescent tracer bound to the target protein-NanoLuc fusion. Compounds applied to cells and specifically engage the intracellular fusion will result in a decrease in BRET. To ensure accurate assessment of intracellular TE, a NanoLuc inhibitor is used to mitigate any extracellular NanoLuc signal that may arise from cells compromised during handling, while not adversely affecting NanoLuc luciferase expressed within healthy living cells.
