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NanoLuc Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independently optimized for stability and minimal self-association. Interaction of the target proteins facilitates subunit complementation to give a bright, luminescent enzyme. The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A constitutively interacting positive control pair and a negative control vector. Starter systems also include the Nano-Glo Live Cell Assay System, a single-addition, non-lytic detection reagent used for monitoring NanoBiT luminescence in living cells.
