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  1. Promega
  2. Cellular & Proteomic Solutions
  3. Cell Analysis

NanoBRET(R) PPI Flexi(R) Starter System
PRON1821
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NanoBRET technology is an improved bioluminescence resonance energy transfer (BRET)-based technology using NanoLuc luciferase as the BRET energy donor and HaloTag protein labeled with NanoBRET 618 fluorescent ligand as the energy acceptor to measure the interaction of two binding partners in live cells. NanoBRET Protein:Protein Interaction (PPI) Assays use NanoLuc Luciferase and HaloTag protein fused to target proteins of interest to enable sensitive, reproducible detection of protein interactions in the natural cellular environment. The use of full-length proteins expressed at low levels enables PPI monitoring and screening studies that reflect true cellular physiology. The NanoBRET PPI Starter Systems provide the vectors required to create NanoLuc Luciferase and HaloTag protein fusions to target proteins of interest, the NanoBRET PPI Positive Control Pair (p53,MDM2) and the NanoBRET Nano-Glo Detection System (contains substrate to generate donor signal and ligand as energy acceptor).

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