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Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcgammaRIIa, FcgammaRI and FcgammaRIIIa. While all three receptors can participate in ADCP, FcgammaRIIa is believed to be the predominant Fcgamma receptor involved in this process. The FcgammaRIIa-H ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcgammaRIIa. The assay consists of Jurkat cells stably expressing human FcgammaRIIa-H (the high-affinity H131 variant) and NFAT-induced luciferase. The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats, and unlike traditional primary cell-based assays, amenable for use in quality-controlled drug development settings.
