
Recombinant full-length human DGKZ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase zeta (DAG kinase zeta) is a member of class IV diacylglycerol kinase family, which contains two zinc fingers, an ATP binding site, and four ankyrin repeats with the MARCKS phosphorylation site, and exhibits the preference for 1,2-diacylglycerols but not for long chain diacylglycerols. DGK zeta regulates Ras activation via terminating the diacylglycerol signaling induced RasGRP. Kinase Enzyme System contains: DGKZ Kinase, 10ug (Human, recombinant, full-length); MW: ~132kDa. Dilauroyl-sn-glycerol Substrate, 10mM. 5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II.