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Recombinant full-length human DGKB was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase beta (DAG kinase beta) is a member of class I diacylglycerol kinase family and is regulated by Ca2+ via converting diacylglycerol into phosphatidic acid, diacylglycerol kinases decrease the diacylglycerol levels to regulate the activity of PKC, Rho and Ras families. DGK beta is localized in brain and is involved in emotion and long-term memory linked to cognitive function. Kinase Enzyme System contains: DGKB Kinase, 10ug (Human, recombinant, full-length); MW: ~118kDa. Dilauroyl-sn-glycerol Substrate, 10mM. 5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II; CaCl2 Solution, 1M.
