CyBio Drop automated liquid handler
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The GITR Bioassay (Cat.# J2272, JA2291, JA2295) is a bioluminescent cell-based assay that reflects the mechanism of action (MOA) of biologics designed to activate GITR. The assay consists of a genetically engineered Jurkat T cell line that expresses human GITR and a luciferase reporter driven by a response element that can respond to GITR ligand/agonist antibody stimulation. Conduct the GITR Bioassay with Fc?RIIb CHO-K1 Cells (Cat.# JA2251, JA2255) to test whether agonist antibodies activate GITR in an Fc?RIIb-dependent manner. Fc?RIIb CHO-K1 cells may be required to crosslink agonist antibodies but are not required for testing ligands. When screening for agonist antibodies of costimulatory immune checkpoints, you should perform the assay both with and without Fc?RIIb CHO-K1 Cells to ascertain the need for these cells in enhancing the effect of the agonist antibodies raised against the costimulatory immune checkpoint targets.
