Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins. CDK6 is a member of the cyclin-dependent family of protein kinases. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. Kinase Enzyme System contains: Kinase: CDK6/CyclinD3, 10ug (Human, recombinant full-length). MW: ~40kDa (CDK6) and ~35kDa (CyclinD3). Substrate: Native Histone H1 Protein; purified from calf thymus tissues. Other: Reaction Buffer, DTT. CDK6/CyclinD3 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1021/.
