Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition (2). Kinase Enzyme System contains: Kinase: CDK2/CyclinE1, 10ug (Human, recombinant full-length). MW: ~58kDa (CDK2) and ~73kDa (CyclinE1). Substrate: Native Histone H1 Protein; purified from calf thymus tissues. Other: Reaction Buffer, DTT. CDK2/CyclinE1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1017/.
