Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported. Kinase Enzyme System contains: Kinase: CDK3/CyclinE1, 10ug (Human, recombinant full-length). MW: ~60kDa (CDK3) and ~73kDa (CyclinE1). Substrate: Native Histone H1 Protein; purified from calf thymus tissues. Other: Reaction Buffer, DTT. CDK3/CyclinE1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1018/.
