Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. A ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family, CAMK2 alpha is abundant in the brain as a major constituent of postsynaptic density and required for hippocampal long-term potentiation and spatial learning. CAMK2 alpha also can undergo autophosphorylation, resulting in Ca2+/calmodulin-independent activity. The protein level of CAMK2 alpha fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2 alpha increased with heightened neuronal activity (2). Kinase Enzyme System contains: Kinase: CAMK2 alpha, 10ug (Human, recombinant full-length). MW: ~74kDa. Substrate: Autocamtide 2 peptide (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acids 283-290) on CaMKII. Other: Reaction Buffer, DTT, Ca2+/Calmodulin solution. CAMK2 alpha NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/815/.
