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The AMP-Glo Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP as a reaction product. This versatile system can measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. The assay is designed to quantitatively monitor the concentration of AMP in a biochemical reaction in a wide range of plate formats, including HT formats. The stable luminescent signal of the assay eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate. The assay has a high dynamic range and produces a strong signal at low substrate conversion, making it well suited for screening low-activity enzymes. The assay produces minimal false hits and Z? values greater than 0.7.
