Bioneer ExiProgen Protein Biosynthesis, DNA/RNA Purification Instrument
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The cAMP-Glo Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on G protein-coupled receptors (GPCRs). The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction. This improved version combines the lysis and cAMP reaction buffers into the cAMP-Glo ONE Buffer. This format streamlines the protocol and reduces the time needed to complete the assay. The ONE Buffer is supplied at a 5X concentration, which provides increased flexibility for starting cell culture volumes. The cAMP-Glo Max Assay can be performed in 96-, 384- or 1536-well plates.
