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Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest. The activity of ZAK plays a role in cell cycle checkpoint regulation as well as being involved in regulating actin organization (1). Expression of kinase-dead ZAK in mouse fibroblasts disrupts actin stress fibers and causes morphologic changes. ZAK can activate JNK through MKK4/MKK7 and ERK5/p38-gamma via MKK3/MKK6. Expression of ZAK increases the population of cells in the G2/M phase of the cell cycle, whereas dominant-negative ZAK attenuated the G2 arrest caused by gamma radiation (2). Kinase Enzyme System contains: Kinase: ZAK, 10ug (Human, recombinant full-length). MW: ~82kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. ZAK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/51776/.
