RACK 80 WELL FL ASTD 5RK/PK 4PK/CS
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. In the brain, PKC gamma is translocated to cell membranes during ischemia and is rapidly removed or degraded during the second otherwise lethal ischemic insult in preconditioned brains. This suggests that ischemic preconditioning enhances downregulation of cell signaling mediated by PKC gamma and may thereby provide neuroprotection. Kinase: PKC gamma, 10ug (Human, recombinant full-length). MW: ~105kDa. Substrate: PKCtide (ERMRPRKRQGSVRRRV); derived from protein kinase C epsilon (amino acid 149-164). Other: Reaction Buffer, DTT, Lipid solution. PKC gamma NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5582/.
