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  2. Cellular & Proteomic Solutions
  3. Cell Analysis

ADP-Glo(TM) Kinase Assay + PKC Delta Kinase Enzyme System
PROV9721
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Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79kDa protein kinase that shows strict dependence on the presence of phospholipids but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all tissues and is a major isoform of PKC expressed in hemopoietic cells. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. Kinase Enzyme System contains: Kinase: PKC delta, 10ug (Human, recombinant full-length). MW: ~104kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109-121). Other: Reaction Buffer, DTT, Lipid solution. PKC delta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5580/.

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