pH 6.86 Buffer Solution (Clear) 480mL
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhMST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20). Endogenous full-length MST1 is activated by a variety of stressful stimuli, accompanied by the secondary appearance of a 36kDa Thr183-phosphorylated, caspase-cleaved catalytic fragment (1). Recombinant MST1 undergoes a robust auto-activation in vitro, mediated by an intramolecular autophosphorylation on the activation loop of an MST dimer. MST1 can initiate apoptosis when transiently overexpressed in mammalian cells. Kinase Enzyme System contains: Kinase: MST1, 10ug (Human, recombinant full-length). MW: ~83kDa. Substrate: Axltide (KKSRGDYMTMQIG); derived from mouse Insulin receptor substrate 1 (amino acids 979-989). Other: Reaction Buffer, DTT. MST1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6789/.
