Bioneer AccuRapid Cell-Free Protein Expression Kit

Recombinant full-length human STK3 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. STK3, also known as MST2, encodes a protein of 491-amino acid which contains an N-terminal catalytic domain characteristic of STKs. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall. RAF1 has been shown to counteract apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2). STK3 is a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand. Kinase Enzyme System contains: STK3 Kinase, 10ug (Human, recombinant, full-length); MW: ~87kDa. Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml). 5X Reaction Buffer; DTT, 0.1M.