Bioneer AccuRapid Cell-Free Protein Expression Kit
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Recombinant human InsR (amino acids 980-1382-end) was expressed in Sf9 insect cells using a baculovirus and an N-terminal GST tag. InsR is the insulin receptor tyrosine kinase involved in insulin signaling, cleaved post-translationally into alpha and beta chains, which are covalently linked. Insulin binding stimulates glucose uptake, maintaining fuel homeostasis and preventing diabetes. Studies show that conditional knockout of insulin receptor substrate 2 (IRS2) in mouse pancreas beta cells and brain regions, including the hypothalamus, increased appetite, lean and fat body mass, linear growth, and insulin resistance leading to diabetes. System contains: Kinase: InsR, 10microg (Human, recombinant; amino acids 980-1382-end). MW: ~70kDa. Substrate: Axltide (KKSRGDYMTMQIG); derived from mouse Insulin receptor substrate 1 (amino acids 979-989). Reaction Buffer, DTT, MnCl2. InsR NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/3643/
