Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human InsR (amino acids 1011-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. InsR is the insulin receptor tyrosine kinase that is involved in insulin signaling. InsR is post-translationally cleaved into two chains, alpha and beta, that are covalently linked. Binding of insulin to the InsR stimulates glucose uptake. Insulin receptor signaling helps to maintain fuel homeostasis and prevent diabetes. Studies have shown that a conditional knockout of insulin receptor substrate 2 (IRS2) in mouse pancreas beta cells and parts of the brain?including the hypothalamus?increased appetite, lean and fat body mass, linear growth, and insulin resistance that progressed to diabetes. Kinase Enzyme System contains: InsR (R1201Q) Kinase, 10ug (Human, recombinant; amino acids 1011-end). MW: ~70kDa. AXLtide Substrate (1mg/ml) (KKSRGDYMTMQIG); derived from mouse Insulin receptor substrate 1 amino acids 979-989,. 5X Reaction Buffer, 2.5M MnCl2, 0.1M DTT.
