Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis (1). JNK regulates the expression of HIPK3 in prostate cancer cells and this contributes to increased resistance to Fas receptor-mediated apoptosis by reducing the interaction between FADD and caspase-8 (2). HIPK3 has been reported to phosphorylate FADD and is implicated in multidrug resistance in a number of tumors. HIPK3 increases transcription factor SF-1 activity leading to increased steroidogenic gene expression in response to cAMP signaling. Kinase Enzyme System contains: Kinase: HIPK3, 10ug (Human, recombinant; amino acids 163-562). MW: ~49kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. HIPK3 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/10114/.
