Bioneer AccuRapid Cell-Free Protein Expression Kit
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Recombinant human HIPK1 (156-555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family. HIPK 1 is a nuclear kinase that phosphorylates homeodomain transcription factors. HIPK1 phosphorylates DAXX, and this leads to its relocalization and subsequent decrease in transcriptional repression activity (1). HIPK1 also interacts with p53 and phosphorylates it on serine residues. HIPK1 expression is elevated in breast cancer cell lines and embryonic fibroblasts from HIPK1-null mice showing more susceptibility to apoptosis induced by DNA damage (2). Kinase Enzyme System contains: Kinase: HIPK1, 10ug (Human, recombinant; amino acids 156-555). MW: ~71kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. HIPK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/204851/.
