Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human EIF2AK2 (252-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK2 is a protein kinase shown to be involved in HIV/gp120-associated neurodegeneration. EIF2AK2 acts as a critical mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases that respond to various forms of environmental stress. Activation of EIF2AK2 leads to its autophosphorylation and then phosphorylation of its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. A novel cross-talk event between the EIF2AKs and p53 has been shown that has implications in cell proliferation and tumorigenesis. Kinase Enzyme System contains: EIF2AK2, 10ug (Human, recombinant; amino acids 252-end). MW: ~64kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Reaction Buffer, DTT. EIF2AK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5610/.
