Bioneer AccuRapid Cell-Free Protein Expression Kit

Recombinant human EIF2AK4 (amino acids 192-1024) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK4 or eukaryotic translation initiation factor 2 alpha kinase 4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. The EIF2AK4 eIF2-alpha kinase regulates fatty-acid homeostasis in the liver during deprivation of an essential amino acid. Kinase Enzyme System contains: EIF2AK4 Kinase, 10ug (Human, recombinant; amino acids 192-1024). MW: ~132kDa. RS Repeat Peptide Substrate (GRSRSRSRSRSRSRSR) (1mg/ml). 5X Reaction Buffer, 0.1M DTT.