Stuart Recirculating Coolers - SRC4, SRC14
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus. A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells. This association is highly specific, and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells. Kinase Enzyme System contains: Kinase: CSK, 10ug (Human, recombinant full-length). MW: ~78kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT, MnCl2. CSK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1445/.
