Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex (M-Phase Promoting Factor) that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer also has been shown. Kinase Enzyme System contains: Kinase: CDK1/CyclinA2, 10ug (Human, recombinant full-length). MW: ~59kDa (CDK1); 78kDa (CyclinA2). Substrate: Histone H1- Native histone H1 purified from calf thymus tissues. Other: Reaction Buffer, DTT. CDK1/CyclinA2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/983/.
