Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCDK1 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer also has been demonstrated. Kinase Enzyme System contains: CDK1/CyclinA1 Kinase, 10ug (Human, full-length recombinant). MW: ~89kDa (CDK1), ~78kDa (CyclinA2). Histone H1 Peptide, (1mg/ml), (GGGPATP-KKAKKL-COOH); derived from human histone H1 between amino acids 152-159. 5X Reaction Buffer, 0.1M DTT.
