Pestle, 0.5ml, Assorted, 400/Case
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhCAMKK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) (1). CAMKKI has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKI (2). Kinase Enzyme System contains: Kinase: CAMKK1, 10ug (Human, recombinant full-length). MW: ~94kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT, Ca2+/Calmodulin Solution. CAMKK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/84254/.
