Bioneer AccuRapid Cell-Free Protein Expression Kit

Recombinant mouse NEK1 (1-495) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK1 or NIMA (never in mitosis gene a)-related kinase 1 is a serine/threonine kinase involved in cell cycle regulation and is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. NEK1 is involved early in the DNA damage response pathway. NEK1 cycles through the nucleus via its nuclear localization and export signals. Kinase Enzyme System contains: NEK1 Kinase, 10ug (Human, recombinant; amino acids 1-495); MW: ~85kDa. Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml). 5X Reaction Buffer; DTT, 0.1M.