Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhMARK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Microtubule affinity-regulating kinase 3 (MARK3) is a member of the PAR-1/MARK kinase family, which play critical roles in polarity and cell cycle control and are regulated by 14-3-3 scaffolding proteins, as well as the LKB1 tumour suppressor kinase and atypical protein kinase C (PKC). MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating CDC2. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways. Kinase Enzyme System contains: MARK3 Kinase, 10ug (Human, full-length recombinant). MW: ~110kDa. CHKtide Substrate (1mg/ml) (KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acid 205-225). 5X Reaction Buffer, 0.1M DTT.
