Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human LIMK1 (285-638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. LIMK1 or LIM domain kinase 1 contains a unique combination of 2 N-terminal LIM domain and a C-terminal protein kinase domain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers that can bind to DNA/RNA as well as mediating protein-protein interactions. LIMK1 is thought to be a component of an intracellular signaling pathway that may be involved in brain development especially development of nerve cells. Kinase Enzyme System contains: LIMK1 Kinase, 10ug (Human, recombinant; amino acids 285-638). MW: ~65kDa. Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml). 5X Reaction Buffer, 0.1M DTT.
