Bioneer ExiProgen Protein Biosynthesis, DNA/RNA Purification Instrument

Transcend tRNA is a precharged, biotin-labeled, lysine tRNA that can be used with eukaryotic or prokaryotic in vitro translation systems to tag a protein for easy detection. The biotin tag is stable for 12 months, both as a regent and after incorporation into protein. Using the Transcend? Non-Radioactive Translation Detection Systems (Promega Cat.# L5070, L5080), as little as 0.5ng of protein can be detected via Western blot using either chemiluminescent or colorimetric techniques. Results can be obtained 3-4 hours after gel electrophoresis.