Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
TNT Coupled Wheat Germ Extract Systems offer an alternative for eukaryotic cell-free protein expression: a one-tube, coupled transcription/translation system, that greatly simplifies the process and reduces the time needed to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6 or T7 RNA polymerase promoters. The standard process requires separate reactions with several steps between each reaction. The TNT Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT Extract reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than standard in vitro wheat germ extract translations using RNA templates. Use Magnesium Acetate and Potassium Chloride to optimize in vitro translation reactions in the TNT T7 Quick Coupled Transcription/Translation, Flexi Rabbit Reticulocyte Lysate and TNT Coupled Wheat Germ Extract Systems.
