Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The LAG-3/MHCII Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the LAG-3/MHCII interaction. The assay consists of two cell lines: LAG-3 Effector Cells, Jurkat T cells stably expressing human LAG-3 and a luciferase reporter driven by T-cell activation pathway-dependent response elements; MHCII APC Cells, APC Cells presenting TCR Activating Antigen (Ag), a proprietary peptide designed to specifically activate the TCR on the LAG-3 Effector Cells. When the two cell types are co-cultured in the presence of Ag, MHCII on the APC cells presents the Ag to the TCR on the LAG-3 Effector Cells to activate the Effector Cells. LAG-3 on the Effector Cells inhibits TCR signaling and promoter-mediated luciferase activity. Addition of the anti-LAG-3 antibody releases LAG-3 mediated inhibition, resulting in promoter-mediated luminescence. Control Ab, Anti-LAG-3, is available separately.
