Harvard Locomotor Activity and Exploration
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Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement, indicating that SLK is needed for microtubule organization and proper localization of Golgi complex (1). Expression of v-Src can downregulate SLK activity through the involvement of CK2, which can directly phosphorylate and inhibit SLK activity, whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates. Kinase Enzyme System contains: Kinase: SLK, 10ug (Human, recombinant full-length). MW: ~180kDa. Substrate: Native Histone H3 Protein; purified from calf thymus tissues. Other: Reaction Buffer, DTT. SLK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/9748/.
